Biological Specification for Planting of Landscape Materials

 The following is required to obtain the best possible survival and growth of plants. It is required that the root systems of the plants have mycorrhizae formed by diverse fungi (multiple species), biostimulants to feed microbial organisms in the soil to promote root growth, the addition of nitrogen fixing, phosphate solubilizing, and growth promoting bacteria, Trichoderma, a natural Trichoderma that also prevents fungal diseases, and a polymer gel to manage water and slows the release of soluble components in the root zone.

 SECTION 1.0 - TRANSPLANTING TREES, SHRUBS AND BARE ROOT PLANTS

 Section 1.1 - Trees & Shrubs: Work shall consist of treating the root ball of all trees and shrubs immediately adjacent to the root ball in the upper 8-10 inches of the planting hole with the manufacturer's recommended amount with (1) mycorrhizal fungal inoculants of diverse endomycorrhizal fungi (10 Propagules per cubic centimeter Glomus mosseae (3), Glomus intraradices (3), Glomus fasciculatum (2), Glomus dussii (1), Glomus clarum (1), Glomus deserticola (1), Glomus microaggregatum (1) and ectomycorrhizal fungi [(1,325,000 spores per cubic centimeter to include:  Pisolithus tinctorius (1,180,000), Rhizopogon (63,500),  Scleroderma (63,500)], Laconia (18,000)), (2) Nitrogen Fixing, Phosphate solubilizing, Cytokinin-producing and Growth Promoting Bacteria [Bacillus (35 species), Pseudomonas (5 species), Streptomyces (7 species)], (3) Trichoderma [Trichoderma (6 species) 1,250,000 Colony Forming Units per cc., (Trichoderma virens (2 strains), Trichoderma harzianum (2 strains), Trichoderma viride, Trichoderma spp.)], (4) organic biostimulants to stimulate microbial activity in the ground and promote root growth, and (5) agricultural grade planting gel (26%) to maintain moisture in the root zone. (Use of DIEHARD™ Transplant (800-628-6373), or equal, meets this specification.) 

Section 1.2 - Bare Root Planting: Work shall consist of dipping the roots in a mixture containing (1) a horticultural grade gel (55%) (300 – 800 micron), (2) mycorrhizal fungal inoculants of diverse Endomycorrhizal fungi (43 Propagules per cubic centimeter Glomus mosseae (9), Glomus intraradices (9), Glomus fasciculatum (9), Glomus dussii (4), Glomus clarum (4), Glomus deserticola (4), Glomus microaggregatum (4) and ectomycorrhizae (1,325,000 spores per cubic centimeter to include:  Pisolithus tinctorius (1,180,000), Rhizopogon (63,500),  Scleroderma (63,500)), Laconia (18,000)), (3) Nitrogen Fixing, Phosphate solubilizing, Cytokinin-producing and Growth Promoting Bacteria. [Bacillus (35 species),  Pseudomonas (5 species), Streptomyces (7 species)], (4) Trichoderma [Trichoderma (6 species), Trichoderma virens (2 strains), Trichoderma harzianum (2 strains), Trichoderma viride, Trichoderma spp.)], and (5) Organic biostimulants to stimulate microbial activity in the ground and promote root growth.  (Use of DIEHARD™ Root Dip (800-628-6373), or equal, meets this specification.)
 

SECTION 2.0 – SHRUB BED SOIL AMENDMENT

 Section 2.1  -  Microbial Soil Amendment:  Work shall consist of amending prepared bedding/planting mix at the rate of 2 pounds per cubic yard with a compound containing (1) mycorrhizal fungal inoculants of diverse fungi, (2) Nitrogen Fixing, Phosphate solubilizing, Cytokinin-producing and Growth Promoting Bacteria. [Bacillus (35 species), Pseudomonas (5 species), Streptomycetes (7 species)], (3) Trichoderma [Trichoderma (6 species), Trichoderma virens (2 strains), Trichoderma harzianum (2 strains), Trichoderma viride, Trichoderma spp.)], (4) Organic biostimulants to stimulate microbial activity in the ground and promote root growth, and (5) Planting gel to maintain moisture in the root zone.

(Use of DIEHARD™ Bed Prep (800-628-6373), or equal, meets this specification.)

 SECTION 3.0 – TURF GRASS – SOD, SPRIGGING, SEEDING

 Section 3.1 – Sod:  This work shall consist of ground preparation before sod is placed by broadcasting at the rate of 3 pounds per 100 sq. ft. worked into the first 2 inches of soil a compound containing (1) mycorrhizal fungal inoculants of diverse fungi, (2) Nitrogen Fixing, Phosphate solubilizing, Cytokinin-producing and Growth Promoting Bacteria. [( Bacillus (35 species),  Pseudomonas (5 species),  Streptomyces (7 species)], (3) Trichoderma [Trichoderma (6 species), Trichoderma virens (2 strains), Trichoderma harzianum (2 strains), Trichoderma viride, Trichoderma spp.), (4) Organic biostimulants to stimulate microbial activity in the ground and promote root growth, and (5) Planting gel to maintain moisture in the root zone. 

(Use of DIEHARD™ Turf (800-628-6373), or equal, meets this specification.)

 Section 3.2 – Sprigging:  This work shall consist of ground preparation before sprigs are cut into the ground by broadcasting at the rate of 3 pounds per 100 sq. ft. worked into the first 2 inches of soil a compound containing (1) mycorrhizal fungal inoculants of diverse fungi, (2) Nitrogen Fixing, Phosphate solubilizing, Cytokinin-producing and Growth Promoting Bacteria. [ Bacillus (35 species), Pseudomonas (5 species),  Streptomyces (7 species)], (3) Trichoderma [Trichoderma (6 species), Trichoderma virens (2 strains), Trichoderma harzianum (2 strains), Trichoderma viride, Trichoderma spp.)], (4) Organic biostimulants to stimulate microbial activity in the ground and promote root growth, and (5) Planting gel to maintain moisture in the root zone. 

(Use of DIEHARD™ Turf (800-628-6373), or equal, meets this specification.)

 Section 3.3 – Seeding:  This work shall consist of ground preparation before seeding by broadcasting at the rate of 3 pounds per 100 sq. ft. worked into the first 2 inches of soil a compound containing (1) mycorrhizal fungal inoculants of diverse fungi, [(10 Propagules per cubic centimeter Glomus mosseae (2), Glomus intraradices (2), Glomus fasciculatum (2), Glomus dussii (1), Glomus clarum (1), Glomus deserticola (1), Glomus microagregatum], (2) Nitrogen Fixing, Phosphate solubilizing, Cytokinin-producing and Growth Promoting Bacteria. [(78,000 Colony Forming Units per cubic centimeter of Bacillus (35 species), Pseudomonas (5 species), Streptomyces (7 species)], (3) Trichoderma [(117,600 Colony Forming Units per cubic centimeter of Trichoderma (6 species), Trichodermavirens (2 strains), Trichoderma harzianum (2 strains), Trichoderma viride, Trichoderma spp.)], (4) organic biostimulants (31%) to stimulate microbial activity in the ground and promote root growth, and (5) planting gel (5%) to maintain moisture in the root zone. 

(Use of DIEHARD™ Turf (800-628-6373), or equal, meets this specification.)

 SECTION 4.0 - RETROFIT FOR ESTABLISHED TREES, TREES IN THE PATH OF CONSTRUCTION, AND NEWLY PLANTED TREES IN STRESS. 

Section 4.1 - Established Trees & Trees in the Path of Construction: This work shall consist of boring 1-2 inch holes and applying 6 oz. of a compound in the area of the active root zone on a 3 foot grid pattern.  Compound shall contain (1) mycorrhizal fungal inoculants of diverse fungi (10 Propagules per cubic centimeter Glomus mosseae (2), Glomus intraradices (2), Glomus fasciculatum (2), Glomus dussii (1), Glomus clarum (1), Glomus deserticola (1), Glomus microagregatum, and 180,000 spores per cubic centimeter Pisolithus tinctorius (158,400), Rhizopogon (9,000),  Scleroderma (9,000), Laconia (3,600), (2) Nitrogen Fixing, Phosphate solubilizing, Cytokinin-producing and Growth Promoting Bacteria. (78,000 Colony Forming Units per cubic centimeter of Bacillus (35 species), Pseudomonas (5 species), Streptomyces (7 species)), (3) Trichoderma (117,600 Colony Forming Units per cubic centimeter of Trichoderma (6 species), Trichoderma virens (2 strains), Trichoderma harzianum (2 strains), Trichoderma viride, Trichoderma spp.), (4) organic biostimulants (31%) to stimulate microbial activity in the ground and promote root growth, and (5) planting gel (5%) to maintain moisture in the root zone.  Placement of compound as follows:

The following placement is suggested: Measure from the trunk to the drip line (A). Divide this number by 2. Measure from the drip line this amount towards the tree and outward from the drip line and treat this area (B) using a grid pattern.

 Section 4.2 - Newly Planted Trees & Shrubs in Stress: Punch holes around the root ball (4 holes per inch caliper). Apply 6 oz. scoop per hole (2" wide x 6-8" deep) and water in. The compound shall consist of: (1) mycorrhizal fungal inoculants of diverse fungi, (2) Nitrogen Fixing, Phosphate solubilizing, Cytokinin-producing and Growth Promoting Bacteria. [(Bacillus (35 species), Pseudomonas (5 species), Streptomyces (7 species)], (3) Trichoderma (Trichoderma (6 species), Trichoderma virens (2 strains), Trichoderma harzianum (2 strains), Trichoderma viride, Trichoderma spp.), (4) Organic biostimulants to stimulate microbial activity in the ground and promote root growth, and (5) Planting gel to maintain moisture in the root zone.  (Use of DIEHARD™ Root Reviver (800-628-6373), or equal, meets this specification.)

 SECTION 5 - FLOWER BED PREPARATION

 Section 5.1 - Where Plants Are Concentrated In Flower Bed Areas:  Work shall consist of amending all beds at the rate of 3 lbs. of compound per 100 sq. ft. mixed to a depth of 4 inches.  Compound to containing (1) mycorrhizal fungal inoculants of diverse fungi, (2) Nitrogen Fixing, Phosphate solubilizing, Cytokinin-producing and Growth Promoting Bacteria. [Bacillus (35 species), Pseudomonas (5 species), Streptomyces (7 species)], (3) Trichoderma (Trichoderma (6 species), Trichoderma virens (2 strains), Trichoderma harzianum (2 strains), Trichoderma viride, Trichoderma spp.), (4) Organic biostimulants to stimulate microbial activity in the ground and promote root growth, and (5) Planting gel to maintain moisture in the root zone.  (Use of DIEHARD™ Flower Bed (800-628-6373), or equal, meets this specification.)